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OnDemand Kits

Guaranteed High Quality Custom Kits Expertly Designed and Developed on Request


Product Features


  • Top quality assays with guaranteed results
  • Designed by ‘in-house’ bioinformatical experts, not databases
  • Scientifically validated in our laboratory
  • Preliminary feasibility confirms if OnDemand assay is likely possible
  • An assay for almost any application

OnDemand Kits are sold for research use only and are not licensed for diagnostic procedures.

 

About This Product


OnDemand Kits are Real-Time PCR assays which we have the capability and capacity to design and develop. They are for ‘hotspot’ target organisms which have particular importance in their individual areas of interest and therefore we understand the importance of developing an assay. The OnDemand Kits can be developed on request and to meet your specific requirements meaning you will be provided with an assay which is of the highest quality and which works for you.

We offer specific qPCR assay development including primer/probe design, PCR mix optimization and assay development to ensure a very effective qPCR reaction. We have already developed a huge range kits as part of our ever expanding genesig catalogue. This service allows for the combination of bioinformatic design and development, in conjunction with laboratory testing and evaluation, to provide personally designed primers to match your exact needs and ensure high levels of sensitivity and specificity.

Upon request of development, one of our expert team members will be in contact shortly to completely understand any specific requirements you may have (incl. exclusivity criteria, etc), and they are happy to talk you through the whole process.

A preliminary investigation of the targets below suggest these organisms can have an effective qPCR assay being developed which would match our high performance criteria of specificity, sensitivity and efficiency.

If you are interested in any of these OnDemand Kits or wish to have a custom kit developed please check out the purchasing section below, or alternatively contact us via email: enquiry@primerdesign.co.uk or telephone: +44 (0)2380 748 830.

 

About / Technical


Our standard assay formats include Double-Dye cleavage probe (e.g. TaqMan® probes) or as primer only kits for use with SYBR green chemistry. If you require something more alternative or sophisticated, please do not hesitate to get in contact to enquire about its feasibility.

Kit Contents

  • OnDemand Advanced Kit: Target primer & probe set, positive control, internal extraction control primer & probe set, internal extraction control template, endogenous control primer & probe set, RNase/DNase free water, template preparation buffer, Feasibility Report, Kit Data Sheet (incl. anchor nucleotide).
  • OnDemand Easy Kit: Target primer & probe set, oasig Lyophilised qPCR Master Mix (DNA or RNA), oasig resuspension buffer, positive control, internal extraction control, RNase/DNase free water, template preparation buffer, 54x genesig q16 reaction tubes, Feasibility Report, Kit Data Sheet (incl. anchor nucleotide).

Ours kits will be supplied packaged, optimized and ready to use. If you are looking for pre-dispensed plates or qPCR arrays please contact us.

 

Resources


OnDemand Advanced Kit Handbook
 
OnDemand Easy Kit Handbook
 
OnDemand Kits Design Request Form

 

Purchasing


Price

Price on application - following the process outline below will help us provide you the best price.

Catalogue No.

When ordering an OnDemand Kit, you firstly need to order a Feasibility Report, either before or during the kit development. This is a complete bioinformatic identification of the target regions which are totally specific to your requirements. Below are those two catalogue nos. required.

  • Feasibility Report: OnDemand-Feas
  • Design & Development (150 reaction kit): OnDemand-Dev

Process Outline

If you are interested any of the organisms below, or on any organism not listed below or in our catalogue, the process is as follows:

  • Download the Design Request Form above, or alternatively this can be emailed on request
  • This will help us understand the specific design requirements and specifications
  • Attach the form and email us on enquiry@primerdesign.co.uk
  • Following receipt of the completed form, our expert Research & Development team will review the request and we aim to respond within 1 working day
  • The service timelines, costings and development process details will be supplied

N.B. If you do not understand the Design Request Form or process, please feel free to get in contact us.

 

FAQ


What do you get at the end of an OnDemand Kit Development?

  1. Validated, optimized and effective qPCR kit
  2. Product datasheet (incl. anchor nucleotide)
  3. Feasibility report
  4. Handbook

How long does the kit development take?
It should take 4-6 weeks per kit for design and development.

Can you order recurrent kits of the same design?
Absolutely, with significant cost reduction and dispatched with days.

What info do you need to design my kit?
Typically a gene name, species, and the NCBI accession number is required.

I’m working on an unusual species. Can you still help?
Yes. If you are working on an unusual species then we will check that good quality sequence data is available. If not we may be able to work from sequence data from a closely related species. Once synthesized, we can then validate on cDNA sent in by you, or if that is not possible then we will work on a synthetic version of the amplicon to validate the performance of the primers.

I’m working on a virus/bacteria/pathogen species. Can you design primers for these?
Yes. We have over 600 genesig pathogen detection kits already available. If your pathogen of interest is not in the list we can develop a new kit for you from scratch. You can check for your target here.

What qPCR machine(s) will these kits work on?
Easy Kit Format: Designed for optimum performance with the genesig q16 and only recommended to be used with this platform. Advanced Kit Format: These kits are designed to be open platform and will work perfectly on any qPCR machine.

How efficient is the priming of your kit?
We guarantee an efficiency of greater than 90%. 

Are your kit primers intron spanning?
Not always: As standard we will design the very best possible primers for the organism of interest regardless of intron/exon boundaries. We do not design primers to be intron spanning as standard as this is restrictive and the most efficient location for the primers may not be on an exon-exon junction. However, if you require your assays to span an intron please let us know and we will design primers to accommodate your request. If detection of gDNA is a concern we recommend the use of Precision DNase to remove contaminating gDNA from your RNA.

Is the kit guaranteed to be specific?
Yes. Thorough analysis is carried out during the design phase to ensure specificity to the target. But the gold standard test is carried out in our wet laboratory where we prove specific amplification of a single product on biologically derived cDNA or synthetic template.


Examples of OnDemand Kits

 

A preliminary investigation of the targets below suggest these organisms can have an effective qPCR assay developed which would match our high performance criteria of specificity, sensitivity and efficiency.

Human-Related Targets

Antibiotic Resistance Markers

  • blaCTX-M (beta-lactamases)
  • blaCTX-M2/M9 (beta-lactamases)
  • blaSHV
  • blaTEM
  • MecA
  • VanA
  • VanB

Gastrointestinal Infections

  • Ancylostoma duodenale
  • Blastocystis hominis
  • Enterococcus caseliflavus
  • Helicobacter pylori
  • Necator americanus

Respiratory Infections

  • Actinobacillus capsulatus
  • Human Parainfluenzavirus 4 (HPIV4B)
  • Human Rhinovirus 9 (HRV9)
  • Human Rhinovirus 29 (HRV29)
  • Simkania negevensis
  • WU Polyomavirus (WUPyV)

Vector-Borne Infections

  • Borrelia afzelii
  • Borrelia garinii
  • Chaoyang virus (ChaoV)

Other Human Pathogens

  • Aureobasidium pullulans
  • Bacillus licheniformis
  • Carnobacterium maltaromaticum
  • Coniothyrium telephii
  • Filifactor alocis
  • Human measles
  • Human Polyomavirus 12 (HPyV12)
  • KI polyomavirus (KIPyV)
  • Lactobacillus sakei / Lactobacillus curvatus
  • Schistosoma haematobium
  • Schistosoma mansoni
  • STL polyomavirus (STLPyV)
  • Tsukamurella inchonensis

Veterinary Targets

  • Ancylostoma duodenale     
  • Bluetongue virus (BTV-8)
  • Cladosporium spp
  • Corynebacterium pseudotuberculosis
  • Enterocytozoon hepatopenaei
  • Grass carp reovirus (GCRV)
  • Mycoplasma iowae
  • Mycoplasma suis
  • Ornithobacterium rhinotracheale
  • Photobacterium phosphoreum
  • Slow bee paralysis virus (SBPV)
  • Vesivirus 2117
  • Wesselsbron virus (WESSV)

Food & Agriculture Targets

Speciation

  • European Eel (Anguilla anguilla)
  • European Plaice (Pleuronectes platessa)
  • House Mouse (Mus musculus)
  • Ostrich (Struthio camelus)
  • Pea (Pisum sativum)
  • Universal Fish

Animal Finder

  • Atlantic Cod (Gadus morhua)
  • Cat (Felis silvestris)
  • Coley (Pollachius virens)
  • Deer (Venison)
  • Dog (Canis lupus familiaris)
  • Donkey (Equus africanus asinus)
  • Duck (Anas platyrhynchos)
  • European Plaice (Pleuronectes platessa)
  • Goat (Capra hircus)
  • Haddock (Melanogrammus aeglefinus)
  • Ostrich (Struthio camelus)
  • Pollock (Pollachius pollachius)
  • Universal Fish
  • Water Buffalo (Bubalus bubalis)
  • Whiting (Merlangius merlangus)
  • Wild Turkey (Meleagris gallopavo)

Pathogen Contamination

  • E.coli Shiga toxin (stx2A)
  • E.coli Shiga toxin (stx2C)
  • Shewanella putrefaciens

GMO and Plant Pathogens

  • Empetrum nigrum
  • Lactobacillus plantarum / Lactobacillus paraplantarum
  • Maize-Bt176
  • Maize-Bt11
  • Maize Dwarf Mosaic Virus (MDMV)
  • Yellow Mealworm Beetle (T.molitor)


If you are interested in any OnDemand Kits please check out the purchasing section above, or alternatively contact us via email: enquiry@primerdesign.co.uk or telephone: +44 (0)2380 748 830.